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KMID : 0613820120220101399
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Journal of Life Science
2012 Volume.22 No. 10 p.1399 ~ p.1406
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Effects of Hizikia fusiforme Extracts on Adipocyte Differentiation and Adipogenesis in 3T3-L1 Preadipocytes
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Choi Eun-Ok
Kim Hyang-Suk
Han Min-Ho
Choi Yung-Hyun
Kim Byung-Woo
Hwang Jin-Ah
Hwang Hye-Jin
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Abstract
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The present study was conducted to evaluate the effects of various extracts of Hizikia fusiforme on the anti-obesity effects in 3T3-L1 preadipocytes. We used H. fusiforme extracts from ethanol (EEHF), dichloromethane (CFHF), ethyl acetate (EAFHF), butanol (BFHF), and water (WFHF). Treatment with these extracts significantly suppressed terminal differentiation of 3T3-L1 preadipocytes in a dose-dependent manner as confirmed by a decrease in lipid droplet content through Oil Red O staining; this effect was higher in WFHF than in other extracts. The concentrations of cellular triglyceride were also reduced in 3T3-L1 cells by exposure with these extracts, especially when compared with the controls. Treatment with 200 μg/ml of WFHF and CFHF caused approximately 42.6% and 23.7% reduction, respectively. In addition, the extracts of H. fusiforme significantly reduced the expression levels of key pro-adipogenic transcription factors, including peroxisome proliferator-activated receptor r (PPARr) and CCAAT/enhancer binding proteins α (C/EBPα) and C/EBPβ as compared with controls. Accordingly, our data indicated that WFHF has a preeminent effect on inhibition of adipocyte differentiation among various extracts, and H. fusiforme extracts may be an ideal candidate for obesity relief.
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KEYWORD
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Hizikia fusiforme, 3T3-L1, obesity, adipogenesis, peroxisome proliferator-activated receptor r (PPARr), CCAAT/enhancer binding protein (C/EBP)
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